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cck 8 solution  (MedChemExpress)


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    MedChemExpress cck 8 solution
    Cck 8 Solution, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 4576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+counting+kit+8+cck+8+solution/Cell+Counting+Kit-8/pm42432728-106-9-12
    Average 99 stars, based on 4576 article reviews
    cck 8 solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: GPR83 protects cochlear hair cells against ibrutinib-induced hearing loss through AKT signaling pathways
    Article Snippet: .. Subsequently, 10 μL of cell counting kit-8 (CCK-8) solution (HY-K0301; MedChemExpress, Monmouth Junction, NJ, United States) was introduced to the cell cultures, which were maintained at 37°C for 30 min. .. The absorbance of the CCK-8 cells was measured using a microplate reader (BioTek Synergy HTX, Agilent, United States).

    Article Title: Lnc_011797 promotes ferroptosis and aggravates white matter lesions
    Article Snippet: .. HUVECs were inoculated into 96-well plates, and 10 μL of cell counting kit-8 (CCK-8) solution (MedChemExpress) was added to each well. .. After the cells were incubated at 37°C for 2 hours, the absorbance at 450 nm was measured with a microplate reader (Molecular Devices, San Jose, CA, USA) (Mao et al., 2019).

    Article Title: Dendrobine attenuates sepsis-associated acute kidney injury by promoting PINK1/PARKIN-mediated mitophagy.
    Article Snippet: .. HK-2 cells were plated in 96-well plates at a density of 7000 cells per well and cultured for 24 h. Following this, the cells were exposed to 10 μL of the cell counting kit-8 (CCK-8) solution (HY-K0301, MedChemExpress) for 2 h at 37 ◦C. ..

    Article Title: Lnc_011797 promotes ferroptosis and aggravates white matter lesions
    Article Snippet: .. HUVECs were inoculated into 96-well plates, and 10 μL of cell counting kit-8 (CCK-8) solution (MedChemExpress) was added to each well. .. After the cells were incubated at 37°C for 2 hours, the absorbance at 450 nm was measured with a microplate reader (Molecular Devices, San Jose, CA, USA) (Mao et al., 2019).

    Article Title: Mechanistic Insights into Luteolin’s Effect on Gastric Cancer: Inhibition of MCL1 and its Impact on PI3K-Akt and JAK-STAT Signaling
    Article Snippet: .. Following incubation, 10 μL of cell counting kit‐8 (CCK‐8) solution (HY‐K0301, MCE) was added to each well and incubated at 37°C for 1–2 hours. ..

    Article Title: Exosomal miR-3126-5p derived from cancer-associated fibroblasts facilitates glycolysis to accelerate NSCLC progression by targeting KLF13 to activate the SH2B1/IRS1 axis.
    Article Snippet: .. After multiple treatments, 15 μL of cell counting kit-8 (CCK-8) solution (HY-K0301, MedChemExpress) was dispensed into each well and then incubated for 2 h of incubation at 37◦C. .. The microplate reader (Thermo Fisher Scientific) was utilized to assess absorbance at 450 nm.

    Article Title: A novel Bruton's tyrosine kinase inhibitor JDB175 shows potent efficacy to suppress central nervous system lymphoma
    Article Snippet: .. After the designated treatment period, 20 μL of cell counting kit‐8 (CCK‐8) solution (MedChemExpress) was added to each well and incubated at 37°C for 2 h. The absorbance at 450 nm was measured using a microplate spectrophotometer (Molecular Devices) and the inhibition rate at each concentration was calculated. .. The IC 50 values were calculated using GraphPad Prism 8 software (GraphPad Inc.).

    Article Title: Exosomal miR‐3126‐5p derived from cancer‐associated fibroblasts facilitates glycolysis to accelerate NSCLC progression by targeting KLF13 to activate the SH2B1/IRS1 axis
    Article Snippet: .. After multiple treatments, 15 μL of cell counting kit‐8 (CCK‐8) solution (HY‐K0301, MedChemExpress) was dispensed into each well and then incubated for 2 h of incubation at 37°C. .. The microplate reader (Thermo Fisher Scientific) was utilized to assess absorbance at 450 nm.

    Cell Culture:

    Article Title: Dendrobine attenuates sepsis-associated acute kidney injury by promoting PINK1/PARKIN-mediated mitophagy.
    Article Snippet: .. HK-2 cells were plated in 96-well plates at a density of 7000 cells per well and cultured for 24 h. Following this, the cells were exposed to 10 μL of the cell counting kit-8 (CCK-8) solution (HY-K0301, MedChemExpress) for 2 h at 37 ◦C. ..

    Incubation:

    Article Title: Mechanistic Insights into Luteolin’s Effect on Gastric Cancer: Inhibition of MCL1 and its Impact on PI3K-Akt and JAK-STAT Signaling
    Article Snippet: .. Following incubation, 10 μL of cell counting kit‐8 (CCK‐8) solution (HY‐K0301, MCE) was added to each well and incubated at 37°C for 1–2 hours. ..

    Article Title: Exosomal miR-3126-5p derived from cancer-associated fibroblasts facilitates glycolysis to accelerate NSCLC progression by targeting KLF13 to activate the SH2B1/IRS1 axis.
    Article Snippet: .. After multiple treatments, 15 μL of cell counting kit-8 (CCK-8) solution (HY-K0301, MedChemExpress) was dispensed into each well and then incubated for 2 h of incubation at 37◦C. .. The microplate reader (Thermo Fisher Scientific) was utilized to assess absorbance at 450 nm.

    Article Title: A novel Bruton's tyrosine kinase inhibitor JDB175 shows potent efficacy to suppress central nervous system lymphoma
    Article Snippet: .. After the designated treatment period, 20 μL of cell counting kit‐8 (CCK‐8) solution (MedChemExpress) was added to each well and incubated at 37°C for 2 h. The absorbance at 450 nm was measured using a microplate spectrophotometer (Molecular Devices) and the inhibition rate at each concentration was calculated. .. The IC 50 values were calculated using GraphPad Prism 8 software (GraphPad Inc.).

    Article Title: Exosomal miR‐3126‐5p derived from cancer‐associated fibroblasts facilitates glycolysis to accelerate NSCLC progression by targeting KLF13 to activate the SH2B1/IRS1 axis
    Article Snippet: .. After multiple treatments, 15 μL of cell counting kit‐8 (CCK‐8) solution (HY‐K0301, MedChemExpress) was dispensed into each well and then incubated for 2 h of incubation at 37°C. .. The microplate reader (Thermo Fisher Scientific) was utilized to assess absorbance at 450 nm.

    Spectrophotometry:

    Article Title: A novel Bruton's tyrosine kinase inhibitor JDB175 shows potent efficacy to suppress central nervous system lymphoma
    Article Snippet: .. After the designated treatment period, 20 μL of cell counting kit‐8 (CCK‐8) solution (MedChemExpress) was added to each well and incubated at 37°C for 2 h. The absorbance at 450 nm was measured using a microplate spectrophotometer (Molecular Devices) and the inhibition rate at each concentration was calculated. .. The IC 50 values were calculated using GraphPad Prism 8 software (GraphPad Inc.).

    Inhibition:

    Article Title: A novel Bruton's tyrosine kinase inhibitor JDB175 shows potent efficacy to suppress central nervous system lymphoma
    Article Snippet: .. After the designated treatment period, 20 μL of cell counting kit‐8 (CCK‐8) solution (MedChemExpress) was added to each well and incubated at 37°C for 2 h. The absorbance at 450 nm was measured using a microplate spectrophotometer (Molecular Devices) and the inhibition rate at each concentration was calculated. .. The IC 50 values were calculated using GraphPad Prism 8 software (GraphPad Inc.).

    Concentration Assay:

    Article Title: A novel Bruton's tyrosine kinase inhibitor JDB175 shows potent efficacy to suppress central nervous system lymphoma
    Article Snippet: .. After the designated treatment period, 20 μL of cell counting kit‐8 (CCK‐8) solution (MedChemExpress) was added to each well and incubated at 37°C for 2 h. The absorbance at 450 nm was measured using a microplate spectrophotometer (Molecular Devices) and the inhibition rate at each concentration was calculated. .. The IC 50 values were calculated using GraphPad Prism 8 software (GraphPad Inc.).



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    cck 8 solution  (Dojindo Labs)
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    Dojindo Labs cck 8 solution
    Effects of miR-32-5p on the proliferative capacities, migratory abilities and apoptotic rate of UCEC cells. (A) The expression of miR-32-5p in HEC-1-A and Ishikawa cells were then determined using quantitative reverse transcription-PCR. ***P<0.001 vs. HEC-1-A cells. (B) The expression of miR-32-5p in HEC-1-A cells after transfection of miR-32-5p inhibitor or in Ishikawa cells after transfection of miR-32-5p mimic. (C) The viability of UCEC cell lines were measured using <t>the</t> <t>CCK-8</t> assay. (D) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (E) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (F) The migratory potential of UCEC cell lines were determined via Transwell migration assay. (Scale bar, 100 µm). (G) Flow cytometry was used to detect apoptosis. * P<0.05, **P<0.01, ***P<0.001 vs. miR-32-5p inhibitor-NC or miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; EdU, 5-ethynyl-2′-deoxyuridine.
    Cck 8 Solution, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+counting+kit+8+cck+8+solution/Cell+Counting+Kit-8/pmc13101041-92-22-26
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    cck 8 solution  (MedChemExpress)
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    MedChemExpress cck 8 solution
    Effects of miR-32-5p on the proliferative capacities, migratory abilities and apoptotic rate of UCEC cells. (A) The expression of miR-32-5p in HEC-1-A and Ishikawa cells were then determined using quantitative reverse transcription-PCR. ***P<0.001 vs. HEC-1-A cells. (B) The expression of miR-32-5p in HEC-1-A cells after transfection of miR-32-5p inhibitor or in Ishikawa cells after transfection of miR-32-5p mimic. (C) The viability of UCEC cell lines were measured using <t>the</t> <t>CCK-8</t> assay. (D) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (E) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (F) The migratory potential of UCEC cell lines were determined via Transwell migration assay. (Scale bar, 100 µm). (G) Flow cytometry was used to detect apoptosis. * P<0.05, **P<0.01, ***P<0.001 vs. miR-32-5p inhibitor-NC or miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; EdU, 5-ethynyl-2′-deoxyuridine.
    Cck 8 Solution, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+counting+kit+8+cck+8+solution/Cell+Counting+Kit-8/pm42432728-106-9-12
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    cck 8 working solution  (Beyotime)
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    Beyotime cck 8 working solution
    In vitro cytocompatibility assessment . <t>(a)</t> <t>CCK-8</t> assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
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    cell counting kit cck 8 solution  (Servicebio Inc)
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    Servicebio Inc cell counting kit cck 8 solution
    SalB attenuated CORT-induced HT22 cell damage. (A) HT22 cells were exposed to 0–250 μM CORT for 24 h, and <t>CCK-8</t> assessed cell viability. (B) HT22 cells were treated with 2.5–40 μM SalB for 24 h, and CCK-8 assessed cell viability. (C) HT22 cells were pretreated with 10, 15 and 20 μM SalB for 1 h, and then stimulated with 200 μM CORT for 24 h. CCK-8 assessed cell viability. SalB significantly increased it. (D) The LDH level of the cells was detected by the kit, which was significantly reduced after SalB intervention. (E, F) Flow cytometry detected apoptosis level, which was significantly reduced after SalB intervention. (G–I) ELISA detected inflammatory factor content. SalB markedly declined TNF-α, IL-1β and IL-6 contents. (J, K) The intracellular ROS level was assessed using flow cytometry, which was markedly lessened after SalB intervention. (L–N) Oxidative stress indexes were detected by kits. SalB significantly increased GSH-Px and SOD activities and decreased MDA contents. n = 3, ** p < 0.01, *** p < 0.001 vs. 0/Control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CORT group.
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    Image Search Results


    Effects of miR-32-5p on the proliferative capacities, migratory abilities and apoptotic rate of UCEC cells. (A) The expression of miR-32-5p in HEC-1-A and Ishikawa cells were then determined using quantitative reverse transcription-PCR. ***P<0.001 vs. HEC-1-A cells. (B) The expression of miR-32-5p in HEC-1-A cells after transfection of miR-32-5p inhibitor or in Ishikawa cells after transfection of miR-32-5p mimic. (C) The viability of UCEC cell lines were measured using the CCK-8 assay. (D) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (E) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (F) The migratory potential of UCEC cell lines were determined via Transwell migration assay. (Scale bar, 100 µm). (G) Flow cytometry was used to detect apoptosis. * P<0.05, **P<0.01, ***P<0.001 vs. miR-32-5p inhibitor-NC or miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; EdU, 5-ethynyl-2′-deoxyuridine.

    Journal: Oncology Letters

    Article Title: Exosomal-miR-32-5p directly targets FOXN2 to regulate the proliferation, migration and apoptosis of uterine corpus endometrial carcinoma via the PI3K/AKT/BCL-2 pathway

    doi: 10.3892/ol.2026.15574

    Figure Lengend Snippet: Effects of miR-32-5p on the proliferative capacities, migratory abilities and apoptotic rate of UCEC cells. (A) The expression of miR-32-5p in HEC-1-A and Ishikawa cells were then determined using quantitative reverse transcription-PCR. ***P<0.001 vs. HEC-1-A cells. (B) The expression of miR-32-5p in HEC-1-A cells after transfection of miR-32-5p inhibitor or in Ishikawa cells after transfection of miR-32-5p mimic. (C) The viability of UCEC cell lines were measured using the CCK-8 assay. (D) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (E) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (F) The migratory potential of UCEC cell lines were determined via Transwell migration assay. (Scale bar, 100 µm). (G) Flow cytometry was used to detect apoptosis. * P<0.05, **P<0.01, ***P<0.001 vs. miR-32-5p inhibitor-NC or miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; EdU, 5-ethynyl-2′-deoxyuridine.

    Article Snippet: HEC-1-A or Ishikawa cells (3×10 3 cells/ml) were grown in 96-well plates for 0, 24, 48 and 72 h before adding the CCK-8 solution (15 μl; Dojindo Laboratories, Inc.).

    Techniques: Expressing, Reverse Transcription, Transfection, CCK-8 Assay, Colony Assay, Proliferation Assay, Transwell Migration Assay, Flow Cytometry, Negative Control

    Exo-miR-32-5p regulates the proliferation, migration and apoptosis of UCEC cells through regulating FOXN2 expression and PI3K/AKT/Bcl-2 pathway. The impact of Exo-miR-32-5p on the proliferation, migration and apoptosis of UCEC cells were examined utilizing a co-culture model. (A) Exo-miR-32-5p expression in HEC-1-A cells transfected with miR-32-5p-inhibitor or in Ishikawa cells transfected with miR-32-5p-mimic was determined through qRT-PCR. (B) The viability of UCEC cell lines were measured using the CCK-8 assay. (C) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (D) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (E) The migratory potential of UCEC cell lines were determined via the Transwell migration assay. (F) Flow cytometry was used to detect apoptosis. (G) The mRNA expression of AKT, PI3K, Bcl-2 and FOXN2 was detected by qRT-PCR. (H) The protein levels of AKT, PI3K, p-PI3K, Bcl-2, FOXN2 and the ratio of p-AKT/AKT were measured by western blotting. **P<0.01, ***P<0.001 vs. Exo-miR-32-5p inhibitor-NC or Exo-miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; NS, not significant; WT, wild-type; MUT, mutant; qRT-PCR, quantitative reverse transcription-PCR; p-, phosphorylated; FOXN2, Forkhead Box N2; EdU, 5-ethynyl-2′-deoxyuridine.

    Journal: Oncology Letters

    Article Title: Exosomal-miR-32-5p directly targets FOXN2 to regulate the proliferation, migration and apoptosis of uterine corpus endometrial carcinoma via the PI3K/AKT/BCL-2 pathway

    doi: 10.3892/ol.2026.15574

    Figure Lengend Snippet: Exo-miR-32-5p regulates the proliferation, migration and apoptosis of UCEC cells through regulating FOXN2 expression and PI3K/AKT/Bcl-2 pathway. The impact of Exo-miR-32-5p on the proliferation, migration and apoptosis of UCEC cells were examined utilizing a co-culture model. (A) Exo-miR-32-5p expression in HEC-1-A cells transfected with miR-32-5p-inhibitor or in Ishikawa cells transfected with miR-32-5p-mimic was determined through qRT-PCR. (B) The viability of UCEC cell lines were measured using the CCK-8 assay. (C) Relative colony formation efficiency of UCEC cell lines was evaluated using the colony formation assay. (D) EdU-positive cells were measured through EdU proliferation assay. (Scale bar, 100 µm). (E) The migratory potential of UCEC cell lines were determined via the Transwell migration assay. (F) Flow cytometry was used to detect apoptosis. (G) The mRNA expression of AKT, PI3K, Bcl-2 and FOXN2 was detected by qRT-PCR. (H) The protein levels of AKT, PI3K, p-PI3K, Bcl-2, FOXN2 and the ratio of p-AKT/AKT were measured by western blotting. **P<0.01, ***P<0.001 vs. Exo-miR-32-5p inhibitor-NC or Exo-miR-32-5p mimic-NC. UCEC, uterine corpus endometrial carcinoma; miRNA, microRNA; NC, negative control; NS, not significant; WT, wild-type; MUT, mutant; qRT-PCR, quantitative reverse transcription-PCR; p-, phosphorylated; FOXN2, Forkhead Box N2; EdU, 5-ethynyl-2′-deoxyuridine.

    Article Snippet: HEC-1-A or Ishikawa cells (3×10 3 cells/ml) were grown in 96-well plates for 0, 24, 48 and 72 h before adding the CCK-8 solution (15 μl; Dojindo Laboratories, Inc.).

    Techniques: Migration, Expressing, Co-Culture Assay, Transfection, Quantitative RT-PCR, CCK-8 Assay, Colony Assay, Proliferation Assay, Transwell Migration Assay, Flow Cytometry, Western Blot, Negative Control, Mutagenesis, Reverse Transcription

    In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Journal: Bioactive Materials

    Article Title: Revolutionizing Mg-based guided bone regeneration mesh derived from endogenous dentoalveolar bone augmentation

    doi: 10.1016/j.bioactmat.2026.04.003

    Figure Lengend Snippet: In vitro cytocompatibility assessment . (a) CCK-8 assay results of MC3T3-E1 cells and HUVECs cultured with mesh extracts. (b) Representative live/dead staining images of MC3T3-E1 cells and HUVECs after 1 and 3 days of culture. (c) Representative phalloidin (cytoskeleton) and DAPI (nucleus) staining images of MC3T3-E1 cells and HUVECs after 24 h of culture. (d) SEM images of MC3T3-E1 cells and HUVECs directly cultured on mesh surfaces for 1 day. (e) Optical images of scratch wound-healing assays for MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. (f) Migration rates of MC3T3-E1 cells cultured in different extracts for 0 h, 12 h, and 24 h. n.s.>0.05, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

    Article Snippet: On days 1, 3, and 5 of culture, the existing culture medium was substituted with 100 μL of CCK-8 working solution (Cell Counting Kit-8, Beyotime Biotech, China), prepared by mixing fresh α-MEM and CCK-8 reagent at a volume ratio of 10:1.

    Techniques: In Vitro, CCK-8 Assay, Cell Culture, Staining, Migration

    SalB attenuated CORT-induced HT22 cell damage. (A) HT22 cells were exposed to 0–250 μM CORT for 24 h, and CCK-8 assessed cell viability. (B) HT22 cells were treated with 2.5–40 μM SalB for 24 h, and CCK-8 assessed cell viability. (C) HT22 cells were pretreated with 10, 15 and 20 μM SalB for 1 h, and then stimulated with 200 μM CORT for 24 h. CCK-8 assessed cell viability. SalB significantly increased it. (D) The LDH level of the cells was detected by the kit, which was significantly reduced after SalB intervention. (E, F) Flow cytometry detected apoptosis level, which was significantly reduced after SalB intervention. (G–I) ELISA detected inflammatory factor content. SalB markedly declined TNF-α, IL-1β and IL-6 contents. (J, K) The intracellular ROS level was assessed using flow cytometry, which was markedly lessened after SalB intervention. (L–N) Oxidative stress indexes were detected by kits. SalB significantly increased GSH-Px and SOD activities and decreased MDA contents. n = 3, ** p < 0.01, *** p < 0.001 vs. 0/Control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CORT group.

    Journal: Frontiers in Neurology

    Article Title: Salvianolic acid B alleviates depression-like behaviors by reducing neuronal injury and promoting neurogenesis in a manner associated with JAK-STAT signaling pathway inhibition

    doi: 10.3389/fneur.2026.1817261

    Figure Lengend Snippet: SalB attenuated CORT-induced HT22 cell damage. (A) HT22 cells were exposed to 0–250 μM CORT for 24 h, and CCK-8 assessed cell viability. (B) HT22 cells were treated with 2.5–40 μM SalB for 24 h, and CCK-8 assessed cell viability. (C) HT22 cells were pretreated with 10, 15 and 20 μM SalB for 1 h, and then stimulated with 200 μM CORT for 24 h. CCK-8 assessed cell viability. SalB significantly increased it. (D) The LDH level of the cells was detected by the kit, which was significantly reduced after SalB intervention. (E, F) Flow cytometry detected apoptosis level, which was significantly reduced after SalB intervention. (G–I) ELISA detected inflammatory factor content. SalB markedly declined TNF-α, IL-1β and IL-6 contents. (J, K) The intracellular ROS level was assessed using flow cytometry, which was markedly lessened after SalB intervention. (L–N) Oxidative stress indexes were detected by kits. SalB significantly increased GSH-Px and SOD activities and decreased MDA contents. n = 3, ** p < 0.01, *** p < 0.001 vs. 0/Control group; # p < 0.05, ## p < 0.01, ### p < 0.001 vs. CORT group.

    Article Snippet: Subsequently, 10 μl of cell counting kit (CCK)-8 solution (G4103, Servicebio, Wuhan, China) was added and incubated for 2 h. The optical density (OD) at 450 nm was detected by a microplate reader (1410101, ThermoFisher Scientific, Waltham, MA, USA), and the cell viability was calculated to determine the optimal administration conditions.

    Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control

    SalB attenuated CORT-induced HT-22 cell injury by JAK-STAT signaling. (A) CCK-8 detected cell viability. RO8191 significantly reduced cell viability. (B) The LDH level of cells was detected by the kit, which was significantly increased after RO8191 intervention. (C, D) Flow cytometry detected apoptosis level, which was significantly increased after RO8191 intervention. (E–G) ELISA detected inflammatory factor content. RO8191 markedly enhanced TNF-α, IL-1β and IL-6 contents. (H–J) Oxidative stress index was detected by kit. RO8191 significantly reduced GSH-Px and SOD activities and increased MDA contents. (K, L) The intracellular ROS level was detected by flow cytometry, which was significantly increased after RO8191 intervention. n = 3, *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. CORT group; & p < 0.05, && p < 0.01 vs. CORT+20 μM SalB group.

    Journal: Frontiers in Neurology

    Article Title: Salvianolic acid B alleviates depression-like behaviors by reducing neuronal injury and promoting neurogenesis in a manner associated with JAK-STAT signaling pathway inhibition

    doi: 10.3389/fneur.2026.1817261

    Figure Lengend Snippet: SalB attenuated CORT-induced HT-22 cell injury by JAK-STAT signaling. (A) CCK-8 detected cell viability. RO8191 significantly reduced cell viability. (B) The LDH level of cells was detected by the kit, which was significantly increased after RO8191 intervention. (C, D) Flow cytometry detected apoptosis level, which was significantly increased after RO8191 intervention. (E–G) ELISA detected inflammatory factor content. RO8191 markedly enhanced TNF-α, IL-1β and IL-6 contents. (H–J) Oxidative stress index was detected by kit. RO8191 significantly reduced GSH-Px and SOD activities and increased MDA contents. (K, L) The intracellular ROS level was detected by flow cytometry, which was significantly increased after RO8191 intervention. n = 3, *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. CORT group; & p < 0.05, && p < 0.01 vs. CORT+20 μM SalB group.

    Article Snippet: Subsequently, 10 μl of cell counting kit (CCK)-8 solution (G4103, Servicebio, Wuhan, China) was added and incubated for 2 h. The optical density (OD) at 450 nm was detected by a microplate reader (1410101, ThermoFisher Scientific, Waltham, MA, USA), and the cell viability was calculated to determine the optimal administration conditions.

    Techniques: CCK-8 Assay, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Control